Monoclonal Anti-PRDX6 antibody produced in mouse
SIGMA/WH0009588M1 - clone 3A10-2A11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-1Cys; Anti-AOP2; Anti-KIAA0106; Anti-MGC46173; Anti-NSGPx; Anti-PRX; Anti-aiPLA2; Anti-p29; Anti-peroxiredoxin 6
Product Type: Chemical
Western Blotting PRDX6 monoclonal antibody, clone S1. Western Blot analysis of PRDX6 expression in MCF-7.
Western Blotting PRDX6 monoclonal antibody, clone 3A10-2A11. Western Blot analysis of PRDX6 expression in human liver.
Western Blotting QC Western Blot detection against Immunogen (50.38 kDa).
ELISA Detection limit for recombinant GST tagged PRDX6 is 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3A10-2A11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC035857   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P30041 |
| Biochem/physiol Actions: | Peroxiredoxin 6 (Prdx6) is a bifunctional enzyme, carrying out both phospholipase A2 (PLA2) activity and glutathione (GSH) peroxidase function. The encoded protein aids in redox regulation of the cell and is implicated in reduction of H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. It might also have a crucial role in the regulation of phospholipid turnover and in antioxidant defense. Prdx6 is highly expressed at leading edges of wounds and aids in wound healing. Deficiency of the enzyme activity is observed in cataracts of the lens. Overexpression of Prdx6 leads to several degenerative CNS (central nervous system) disorders such as Alzheimer′s, Parkinson′s, Pick′s, and Crutzfeld-Jakob diseases and schizophrenia. In addition, elevated expression of the enzyme is also observed in various neoplastic diseases including malignant mesothelioma, bronchogenic squamous cell carcinoma and cancer of the gingivo-buccal area, bladder and breast. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Peroxiredoxin 6 (Prdx6) is a non-seleno peroxidase enzyme, encoded by the gene mapped to human chromosome 1q25.1. Prdx6 belongs to thiol-specific antioxidant protein family. It is highly expressed in lung, alveolar epithelial type II cells and alveolar macrophages. The protein is characterized with a single conserved cysteine residue and is called 1-Cys Prdx. Prdx6 contains 224 amino acids. |
| Immunogen: | PRDX6 (AAH35857.1, 1 a.a. ~ 224 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPGGLLLGDVAPNFEANTTVGRIRF |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |