Monoclonal Anti-PPM1F antibody produced in mouse
SIGMA/WH0009647M1 - clone 2A9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CaMKPase; Anti-FEM2; Anti-KIAA0015; Anti-POPX2; Anti-hFEM2; Anti-protein phosphatase 1F (PP2C domain containing)
Product Type: Chemical
Western Blotting Western Blot analysis of PPM1F expression in transfected 293T cell line by PPM1F monoclonal antibody, clone 2A9. Lanes Lane 1: PPM1F transfected lysate (49.8 kDa). Lane 2: Non-transfected lysate.
Western Blotting PPM1F monoclonal antibody, clone 2A9 Western Blot analysis of PPM1F expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged PPM1F is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2A9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_014634   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P49593 |
| General description: | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-depend |
| Immunogen: | PPM1F (NP_055449, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSSGAPQKSSPMASGAEETPGFLDT |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |