Monoclonal Anti-ESPL1 antibody produced in mouse
SIGMA/WH0009700M1 - clone 6H6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ESP1; Anti-KIAA0165; Anti-SEPARIN; Anti-extra spindle poles like 1 (S. cerevisiae)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to ESPL1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 μg/mL]
Western Blotting ESPL1 monoclonal antibody, clone 6H6 Western Blot analysis of ESPL1 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (38.5 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6H6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_012291   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q14674 |
| General description: | Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 604359) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM |
| Immunogen: | ESPL1 (NP_036423, 586 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence REELQAYKAVRADTGQERFNIICDL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |