Monoclonal Anti-TOMM20 antibody produced in mouse
SIGMA/WH0009804M1 - clone 4F3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-KIAA0016; Anti-MAS20; Anti-MGC117367; Anti-MOM19; Anti-TOM20; Anti-translocase of outer mitochondrial membrane 20 homolog (yeast)
MDL Number: MFCD04118702
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Mouse Tmem135 mutation reveals a mechanism involving mitochondrial dynamics that leads to age-dependent retinal pathologies. By: Lee, W. H., Higuchi, H., et al. in Elife, 2016. PubMed ID: 27863209 Image collected and cropped by CiteAb from the following publication, (https://elifesciences.org/articles/19264), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Immunoperoxidase of monoclonal antibody to TOMM20 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to TOMM20 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting TOMM20 monoclonal antibody, clone 4F3. Western Blot analysis of TOMM20 expression in PC-12.
Western Blotting Western Blot analysis of TOMM20 expression in transfected 293T cell line by TOMM20 monoclonal antibody, clone 4F3. Lanes Lane 1: TOMM20 transfected lysate (16.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting TOMM20 monoclonal antibody, clone 4F3 Western Blot analysis of TOMM20 expression in HeLa.
Western Blotting TOMM20 monoclonal antibody, clone 4F3. Western Blot analysis of TOMM20 expression in NIH/3T3.
Western Blotting TOMM20 monoclonal antibody, clone 4F3. Western Blot analysis of TOMM20 expression in rat testis.
Western Blotting TOMM20 monoclonal antibody, clone 4F3. Western Blot analysis of TOMM20 expression in rat brain.
Western Blotting QC Western Blot detection against Immunogen (41.69 kDa).
ELISA Detection limit for recombinant GST tagged TOMM20 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC066335   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q15388 |
| Biochem/physiol Actions: | Translocase of outer mitochondrial membrane 20 (TOMM20) is a subunit of multiple component- dynamic complex, which plays a vital role in importing certain cytosolic proteins into or through the outer membrane of the mitochondria. It acts as a receptor for precursor proteins containing N-terminal cleavable presequences targeted to the mitochondria. Nuclear respiratory factor 2(NRF-2) plays an essential role in regulating transcription of the human TOMM20 gene. TOMM20 has potential as a prognostic biomarker and therapeutic target for gastric cancer. TOMM20 serve as a marker for mitochondrial protein import in inner ear, and reduced expression of TOMM20 is associated with Meniere′s disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Translocase of outer mitochondrial membrane 20 (TOMM20) is a 20kb gene with five exons and four introns, and is mapped to human chromosome 1q42.3. This gene codes for a TOMM20 receptor, which is a subunit of the outer mitochondrial membrane translocase. TOMM20 receptor contains a membrane anchor domain and a cytosolic domain, and is predominantly expressed in human cochlea. |
| Immunogen: | TOMM20 (AAH66335, 1 a.a. ~ 145 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MVGRNSAIAAGVCGALFIGYCIYFD |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |