Monoclonal Anti-GOLGA5 antibody produced in mouse
SIGMA/WH0009950M1 - clone 6B3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-RFG5; Anti-golgi autoantigen, golgin subfamily a, 5; Anti-retII
Product Type: Chemical
Western Blotting GOLGA5 monoclonal antibody, clone 6B3 Western Blot analysis of GOLGA5 expression in K-562.
Enhanced Validation-RNAi Western blot analysis of GOLGA5 over-expressed 293 cell line, cotransfected with GOLGA5 Validated Chimera RNAi. Blot probed with GOLGA5 monoclonal antibody, clone 6B3. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of GOLGA5 expression in transfected 293T cell line by GOLGA5 monoclonal antibody, clone 6B3. Lanes Lane 1: GOLGA5 transfected lysate (83 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.52 kDa).
ELISA Detection limit for recombinant GST tagged GOLGA5 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6B3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_005113   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q8TBA6 |
| General description: | The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. (provided by RefSeq) |
| Immunogen: | GOLGA5 (NP_005104, 2 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SWFVDLAGKAEDLLNRVDQGAATAL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |