Monoclonal Anti-ZBTB33 antibody produced in mouse
SIGMA/WH0010009M1 - clone 2B2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ZNF348; Anti-ZNFkaiso; Anti-zinc finger and BTB domain containing 33
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to ZBTB33 on HeLa cell. [antibody concentration 10 μg/mL]
Enhanced Validation-RNAi Western blot analysis of ZBTB33 over-expressed 293 cell line, cotransfected with ZBTB33 Validated Chimera RNAi. Blot probed with ZBTB33 monoclonal antibody, clone 2B2. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of ZBTB33 expression in transfected 293T cell line by ZBTB33 monoclonal antibody, clone 2B2. Lanes Lane 1: ZBTB33 transfected lysate (74.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
ELISA Detection limit for recombinant GST tagged ZBTB33 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2B2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC042753   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q86T24 |
| General description: | KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA (Filion et al., 2006 [PubMed 16354688]).[supplied by OMIM |
| Immunogen: | ZBTB33 (AAH42753, 564 a.a. ~ 673 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence QFMSSHIKSVHSQDPSGDSKLYRLH |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |