Monoclonal Anti-ACTR2 antibody produced in mouse
SIGMA/WH0010097M1 - clone 1B10-C4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ARP2; Anti-ARP2 actin-related protein 2 homolog (yeast)
MDL Number: MFCD03791942
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to ACTR2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 μg/mL]
Western Blotting ACTR2 monoclonal antibody, clone 1B10-C4. Western Blot analysis of ACTR2 expression in A-431.
Western Blotting ACTR2 monoclonal antibody, clone 1B10-C4 Western Blot analysis of ACTR2 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (69.08 kDa).
ELISA Detection limit for recombinant GST tagged ACTR2 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B10-C4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC014546   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P61160 |
| Biochem/physiol Actions: | ARP2 actin related protein 2 homolog (ACTR2) is a key component of the Arp2/3 complex. It is required for the regulation of the actin cytoskeleton. It plays a vital role in various processes such as cell locomotion and intracellular motility of lipid vesicles. In addition, It is also involved in the regulation of axon guidance and growth cone filopodia formation in Caenorhabditis elegans and Drosophila. |
| General description: | ARP2 actin related protein 2 homolog (ACTR2) is encoded by the gene mapped to human chromosome 2p14. |
| General description: | The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | ACTR2 (AAH14546.1, 1 a.a. ~ 394 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDSQGRKVVVCDNGTGFVKCGYAGS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |