Monoclonal Anti-G3BP antibody produced in mouse
SIGMA/WH0010146M1 - clone 2F3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HDHVIII; Anti-
MDL Number: MFCD02262927
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to G3BP on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 1 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to G3BP on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting G3BP monoclonal antibody, clone 2F3 Western Blot analysis of G3BP expression in A-431.
Enhanced Validation-RNAi Western blot analysis of G3BP over-expressed 293 cell line, cotransfected with G3BP Validated Chimera RNAi. Blot probed with G3BP monoclonal antibody, clone 2F3. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of G3BP expression in transfected 293T cell line by G3BP monoclonal antibody, clone 2F3. Lanes Lane 1: G3BP transfected lysate (52.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (35.53 kDa).
ELISA Detection limit for recombinant GST tagged G3BP1 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC006997   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q13283 |
| Biochem/physiol Actions: | G3BP1 (Ras-GTPase-activating protein SH3 domain-binding protein 1) blocks the replication of HIV-1 (human immunodeficiency virus) in macrophages and T-cells. In gastric cancer patients, overexpression of G3BP1 leads to imperfect clinical predictions. It is crucial for the interactions of SG–PB (stress granule-processing bodies). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | G3BP1 (Ras-GTPase-activating protein SH3 domain-binding protein 1) is a constituent of stress granules. It is located on human chromosome 5q14.2-5q33.3. |
| Immunogen: | G3BP (AAH06997, 214 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KPEPVLEETAPEDAQKSSSPAPADI |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |