Monoclonal Anti-TRIM28 antibody produced in mouse
SIGMA/WH0010155M1 - clone 4E6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-KAP1; Anti-RNF96; Anti-TF1B; Anti-TIF1B; Anti-tripartite motif-containing 28
MDL Number: MFCD02263554
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to TRIM28 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting TRIM28 monoclonal antibody, clone 4E6 Western Blot analysis of TRIM28 expression in HeLa S3 NE.
Enhanced Validation-RNAi Western blot analysis of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi. Blot probed with TRIM28 monoclonal antibody (M01) clone 4E6. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody, clone 4E6. Lanes Lane 1: TRIM28 transfected lysate (88.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting TRIM28 monoclonal antibody, clone 4E6. Western Blot analysis of TRIM28 expression in NIH/3T3.
Western Blotting TRIM28 monoclonal antibody, clone 4E6. Western Blot analysis of TRIM28 expression in PC-12.
Western Blotting QC Western Blot detection against Immunogen (41.69 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4E6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC004978   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q13263 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. (provided by RefSeq) |
| Immunogen: | TRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence IVDPVEPHGEMKFQWDLNAWTKSAE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |