Monoclonal Anti-OLIG2 antibody produced in mouse
SIGMA/WH0010215M2 - clone 3D7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-BHLHB1; Anti-OLIGO2; Anti-PRKCBP2; Anti-RACK17; Anti-oligodendrocyte lineage transcription factor 2
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to OLIG2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of OLIG2 expression in transfected 293T cell line by OLIG2 monoclonal antibody, clone 3D7. Lanes Lane 1: OLIG2 transfected lysate (32.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (34.47 kDa).
Immunoprecipitation Immunoprecipitation of OLIG2 transfected lysate using anti-OLIG2 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with OLIG2 rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3D7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_005806   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q13516 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. (provided by RefSeq) |
| Immunogen: | OLIG2 (NP_005797, 2 a.a. ~ 78 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence DSDASLVSSRPSSPEPDDLFLPARS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |