Monoclonal Anti-M6PRBP1 antibody produced in mouse
SIGMA/WH0010226M1 - clone 2F7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MGC11117; Anti-MGC2012; Anti-PP17; Anti-TIP47; Anti-
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to PLIN3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of PLIN3 expression in transfected 293T cell line by PLIN3 monoclonal antibody, clone 2F7. Lanes Lane 1: PLIN3 transfected lysate (Predicted MW: 47 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (73.48 kDa).
Immunoprecipitation Immunoprecipitation of PLIN3 transfected lysate using anti-PLIN3 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PLIN3 monoclonal antibody.
ELISA Detection limit for recombinant GST tagged PLIN3 is 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC001590   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL |
| General description: | Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene |
| Immunogen: | PLIN3 (AAH01590, 1 a.a. ~ 434 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSADGAEADGSTQVTVEEPVQQPSV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |