Monoclonal Anti-HRSP12 antibody produced in mouse
SIGMA/WH0010247M1 - clone 2B8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-P14.5; Anti-PSP; Anti-UK114; Anti-heat-responsive protein 12
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to HRSP12 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting QC Western Blot detection against Immunogen (40.81 kDa).
ELISA Detection limit for recombinant GST tagged HRSP12 is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2B8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC010280   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL |
| Biochem/physiol Actions: | HRSP12 (Heat-responsive protein 12) mainly acts as a translational inhibitor protein. It has been proposed that the protein may have the potentiality to perform in various cellular mechanisms such as a differentiation-dependent regulation of protein synthesis in hepatocytes, renal tubular epithelial cells, smooth muscle cells and mononuclear phagocytes (MNP). |
| General description: | HRSP12 (Heat-responsive protein 12) is a trichloroacetic acid soluble protein belonging to the hypothetical family of small proteins, termed as YER057c/YJGF family. It has a molecular weight of 14.5kDa. It is expressed in monocytes, liver and kidney tumor cells. Its high level of expression has been reported in fully differentiated monocytes. |
| Immunogen: | HRSP12 (AAH10280.1, 1 a.a. ~ 137 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSSLIRRVISTAKAPGAIGPYSQAV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |