Monoclonal Anti-AKAP8 antibody produced in mouse
SIGMA/WH0010270M1 - clone 3D4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-A kinase (PRKA) anchor protein 8; Anti-AKAP95; Anti-DKFZp586B1222
MDL Number: MFCD01633003
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to AKAP8 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to AKAP8 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of AKAP8 expression in transfected 293T cell line by AKAP8 monoclonal antibody, clone 3D4. Lanes Lane 1: AKAP8 transfected lysate (76.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting AKAP8 monoclonal antibody, clone 3D4 Western Blot analysis of AKAP8 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (38.06 kDa).
Immunoprecipitation Immunoprecipitation of AKAP8 transfected lysate using anti-AKAP8 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with AKAP8 monoclonal antibody.
ELISA Detection limit for recombinant GST tagged AKAP8 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3D4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_005858   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O43823 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA. (provided by RefSeq) |
| Immunogen: | AKAP8 (NP_005849, 551 a.a. ~ 662 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence VDPEMEGDDNLGGEDKKETPEEVAA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |