Monoclonal Anti-KNTC2 antibody produced in mouse
SIGMA/WH0010403M1 - clone 1A10, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HEC; Anti-HEC1; Anti-kinetochore associated 2
MDL Number: MFCD02263005
Product Type: Chemical
Western Blotting KNTC2 monoclonal antibody, clone 1A10 Western Blot analysis of KNTC2 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (36.19 kDa).
ELISA Detection limit for recombinant GST tagged KNTC2 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1A10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC035617   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O14777 |
| General description: | HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM |
| Immunogen: | KNTC2 (AAH35617, 545 a.a. ~ 642 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence TVNQGLSEAMNELDAVQREYQLVVQ |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |