Monoclonal Anti-YAP1 antibody produced in mouse
SIGMA/WH0010413M1 - clone 2F12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-YAP; Anti-YAP2; Anti-YAP65; Anti-Yes-associated protein 1, 65kDa
MDL Number: MFCD04118707
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to YAP1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to YAP1 on HeLa cell. [antibody concentration 30 μg/mL]
Western Blotting Western Blot analysis of YAP1 expression in transfected 293T cell line by YAP1 monoclonal antibody, clone 2F12. Lanes Lane 1: YAP1 transfected lysate (Predicted MW: 54.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting YAP1 monoclonal antibody, clone 2F12. Western Blot analysis of YAP1 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_006106   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | ELISA: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P46937 |
| Application: | Monoclonal Anti-YAP1 antibody produced in mouse has been used in western blotting and co-immunoprecipitation. |
| Biochem/physiol Actions: | Yes-associated protein 1 (YAP1) promotes cell and tissue growth. It interacts with receptor tyrosine-protein kinase (ErbB4) receptor and regulates transcription. YAP1 is an oncogenic protein and contributes to tumor progression. It is highly expressed in hedgehog-associated medulloblastomas and mutations in YAP1 is also implicated in optic fissure closure defects. Knockdown of YAP1 gene has been shown to lead to apoptosis of prostate cancer cells and is considered as a potential target for treatment. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. (provided by RefSeq) |
| General description: | Yes-associated protein 1 (YAP1) is a transcriptional coactivator. It possesses two WW domains, a TID domain (TEA domain family member 1 transcription factor interacting-domain), sarcoma homology 3 domain (SH3) binding motif, a proline rich region and a PDZ motif. It is expressed in eight isoforms. The YAP1 gene is localized on human chromosome 11q22.1. |
| Immunogen: | YAP1 (NP_006097, 53 a.a. ~ 161 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence QIVHVRGDSETDLEALFNAVMNPKT |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |