Monoclonal Anti-RNASEH2A antibody produced in mouse
SIGMA/WH0010535M1 - clone 3G5-F5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-JUNB; Anti-RNASEHI; Anti-RNHIA; Anti-RNHL; Anti-ribonuclease H2, large subunit
Product Type: Chemical
Western Blotting RNASEH2A monoclonal antibody, clone 3G5-F5 Western Blot analysis of RNASEH2A expression in Jurkat.
Western Blotting Western Blot analysis of RNASEH2A expression in transfected 293T cell line by RNASEH2A monoclonal antibody, clone 3G5-F5. Lanes Lane 1: RNASEH2A transfected lysate (33.4 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of RNASEH2A over-expressed 293 cell line, cotransfected with RNASEH2A Validated Chimera RNAi. Blot probed with RNASEH2A monoclonal antibody, clone 3G5-F5. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (58.63 kDa).
ELISA Detection limit for recombinant GST tagged RNASEH2A is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3G5-F5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC011748   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O75792 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid |
| Immunogen: | RNASEH2A (AAH11748, 1 a.a. ~ 299 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDLSELERDNTGRCRLSSPVPAVCR |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |