Monoclonal Anti-PDLIM5 antibody produced in mouse
SIGMA/WH0010611M1 - clone 3E11-F6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ENH; Anti-L9; Anti-LIM; Anti-PDZ and LIM domain 5
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PDLIM5 on A-431 cell. [antibody concentration 10 μg/mL]
Western Blotting PDLIM5 monoclonal antibody, clone 3E11-F6. Western Blot analysis of PDLIM5 expression in HepG2.
Western Blotting QC Western Blot detection against Immunogen (91.3 kDa).
ELISA Detection limit for recombinant GST tagged PDLIM5 is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3E11-F6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC008741   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q96HC4 |
| Biochem/physiol Actions: | PDLIM5 (PDZ and LIM domain 5) is involved in the several cellular processes such as cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. It also acts as a scaffold protein that helps to link protein kinases and transcription factors with cytoskeletal elements. Nonsynonymous polymorphisms of the PDLIM5 gene cause both bipolar disorder (BPD) and schizophrenia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized. (provided by RefSeq) |
| Immunogen: | PDLIM5 (AAH08741, 1 a.a. ~ 596 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSNYSVSLVGPAPWGFRLQGGKDFN |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |