Monoclonal Anti-TRIM16 antibody produced in mouse
SIGMA/WH0010626M1 - clone 5F4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-EBBP; Anti-tripartite motif-containing 16
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of TRIM16 over-expressed 293 cell line, cotransfected with TRIM16 Validated Chimera RNAi. Blot probed with TRIM16 monoclonal antibody, clone 5F4. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of TRIM16 expression in transfected 293T cell line by TRIM16 monoclonal antibody, clone 5F4. Lanes Lane 1: TRIM16 transfected lysate (64 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).
Immunoprecipitation Immunoprecipitation of TRIM16 transfected lysate using anti-TRIM16 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with TRIM16 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged TRIM16 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5F4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_006470   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O95361 |
| Biochem/physiol Actions: | The gene TRIM16 (tripartite motif containing 16), also referred to as estrogen-responsive B box protein (EBBP), encodes a protein that has the ability to heterodimerize with other TRIM proteins and also has E3 ubiquitin ligase activity. It is found to positively regulate transcriptional regulator of the retinoic acid receptor β2 (RARβ2) gene. Overexpression of trim16 facilitates retinoid-induced differentiation, reduces neuroblastoma cell growth, migration, and considerably decreases tumorigenicity in vivo. |
| General description: | This gene was identified as an estrogen and anti-estrogen regulated gene in epithelial cells stably expressing estrogen receptor. The protein encoded by this gene contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. The proteins of this family have been reported to be involved in a variety of biological processes including cell growth, differentiation and pathogenesis. Expression of this gene was detected in most tissues. Its function, however, has not yet been determined. (provided by RefSeq) |
| Immunogen: | TRIM16 (NP_006461, 165 a.a. ~ 273 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LDAARRDKEAELQCTQLDLERKLKL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |