Monoclonal Anti-SIX2 antibody produced in mouse
SIGMA/WH0010736M1 - clone 3D7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-sine oculis homeobox homolog 2 (Drosophila)
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of SIX2 over-expressed 293 cell line, cotransfected with SIX2 Validated Chimera RNAi. Blot probed with SIX2 monoclonal antibody, clone 3D7. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of SIX2 expression in transfected 293T cell line by SIX2 monoclonal antibody, clone 3D7. Lanes Lane 1: SIX2 transfected lysate (32.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (57.75 kDa).
ELISA Detection limit for recombinant GST tagged SIX2 is 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3D7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC024033   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9NPC8 |
| General description: | This gene is a member of the vertebrate gene family which encode proteins homologous to the Drosophila ′sine oculis′ homeobox protein. The encoded protein is a transcription factor which, like other members of this gene family, may be involved in limb or eye development. (provided by RefSeq) |
| Immunogen: | SIX2 (AAH24033, 1 a.a. ~ 291 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSMLPTFGFTQEQVACVCEVLQQGG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |