Monoclonal Anti-WDR4 antibody produced in mouse
SIGMA/WH0010785M1 - clone 1F9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-WD repeat domain 4
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to WDR4 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1 μg/mL]
Western Blotting Western Blot analysis of WDR4 expression in transfected 293T cell line by WDR4 monoclonal antibody, clone 1F9. Lanes Lane 1: WDR4 transfected lysate (Predicted MW: 45.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting WDR4 monoclonal antibody, clone 1F9 Western Blot analysis of WDR4 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (55 kDa).
ELISA Detection limit for recombinant GST tagged WDR4 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC001074   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P57081 |
| General description: | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Two transcript variants encoding the same protein have been found for this gene. (provided by RefSeq) |
| Immunogen: | WDR4 (AAH01074, 1 a.a. ~ 266 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MLLDVAVSPDDRFILTADRDEKIRV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |