Monoclonal Anti-MTHFD2 antibody produced in mouse
SIGMA/WH0010797M1 - clone 4G7-2G3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-NMDMC; Anti-
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to MTHFD2 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3 μg/mL]
Western Blotting MTHFD2 monoclonal antibody, clone 4G7-2G3 Western Blot analysis of MTHFD2 expression in HepG2.
Western Blotting Western Blot analysis of MTHFD2 expression in transfected 293T cell line by MTHFD2 monoclonal antibody, clone 4G7-2G3. Lanes Lane 1: MTHFD2 transfected lysate (26.8 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (61.93 kDa).
ELISA Detection limit for recombinant GST tagged MTHFD2 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4G7-2G3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC001548   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P13995 |
| Biochem/physiol Actions: | Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 (MTHFD2) facilitates the regulation of breast cancer cell migration and invasion. MTHFD2 is involved in mitochondrial one-carbon folate metabolism. Decrease in MTHFD2 expression leads to impairment of cell migration and invasion into extracellular matrix and reduces the cell fraction with a high CD44 expression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 (MTHFD2) is a mitochondrial bifunctional enzyme. It possesses a methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase domain. The gene encoding this protein is localized on human chromosome 2p13.1. |
| Immunogen: | MTHFD2 (AAH01548, 16 a.a. ~ 344 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SLRLRPFHLAAVRNEAVVISGRKLA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |