Monoclonal Anti-FGL2 antibody produced in mouse
SIGMA/WH0010875M1 - clone 6D9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-T49; Anti-fibrinogen-like 2; Anti-pT49
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to FGL2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 0.5 μg/mL]
Western Blotting FGL2 monoclonal antibody, clone 6D9. Western Blot analysis of FGL2 expression in Raw 264.7.
Western Blotting Western Blot analysis of FGL2 expression in transfected 293T cell line by FGL2 monoclonal antibody, clone 6D9. Lanes Lane 1: FGL2 transfected lysate (50 kDa). Lane 2: Non-transfected lysate.
Western Blotting FGL2 monoclonal antibody, clone 6D9 Western Blot analysis of FGL2 expression in HeLa.
Western Blotting FGL2 monoclonal antibody, clone 6D9. Western Blot analysis of FGL2 expression in PC-12.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
Immunoprecipitation Immunoprecipitation of FGL2 transfected lysate using anti-FGL2 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with FGL2 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged FGL2 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6D9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_006682   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q14314 |
| Application: | Monoclonal Anti-FGL2 antibody produced in mouse has been used in immunohistochemistry. |
| Biochem/physiol Actions: | Fibrinogen-like protein 2 (FGL2) exhibits prothrombinase activity. It also shows immune regulatory activity during allograft rejection, abortion, and viral infections. This protein acts as an immune coagulant that produces thrombin directly. FGL2 through the clotting-dependent pathway may play a role in tumor angiogenesis and metastasis. It is involved in the pathogenesis of T helper type 1 (Th1) cytokine-induced fetal loss syndrome and viral-induced fulminant hepatitis. FGL2 gene is involved in modulating regulatory T cells (Treg) function. This protein regulates innate and adaptive immunity. FGL2 may serve as a therapeutic agent for glioblastoma (GBM) by promoting GBM progression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Fibrinogen-like protein 2 (FGL2) belongs to the fibrinogen-like protein family. The FGL2 gene is located on the human chromosome at 7q11.23. |
| Immunogen: | FGL2 (NP_006673, 24 a.a. ~ 123 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence NNETEEIKDERAKDVCPVRLESRGK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |