Monoclonal Anti-PRDX3 antibody produced in mouse
SIGMA/WH0010935M1 - clone 1E3-B2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-AOP1; Anti-MER5; Anti-MGC24293; Anti-PRO1748; Anti-Peroxiredoxin 3; Anti-SP22
MDL Number: MFCD03096615
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PRDX3 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of PRDX3 expression in transfected 293T cell line by PRDX3 monoclonal antibody, clone 1E3-B2. Lanes Lane 1: PRDX3 transfected lysate (Predicted MW: 27.7 kDa). Lane 2: Non-transfected lysate.
Immunoprecipitation Immunoprecipitation of PRDX3 transfected lysate using anti-PRDX3 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PRDX3 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged PRDX3 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1E3-B2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC002685   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P30048 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | PRDX3 (AAH02685, 1 a.a. ~ 256 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAAAVGRLLRASVARHVSAIPWGIS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |