Monoclonal Anti-KIF2C antibody produced in mouse
SIGMA/WH0011004M1 - clone 1G2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-KNSL6; Anti-MCAK; Anti-kinesin family member 2C
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to KIF2C on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to KIF2C on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of KIF2C expression in transfected 293T cell line by KIF2C monoclonal antibody, clone 1G2. Lanes Lane 1: KIF2C transfected lysate (81.3 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of KIF2C over-expressed 293 cell line, cotransfected with KIF2C Validated Chimera RNAi. Blot probed with KIF2C monoclonal antibody, clone 1G2. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting KIF2C monoclonal antibody, clone 1G2 Western Blot analysis of KIF2C expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
Immunoprecipitation Immunoprecipitation of KIF2C transfected lysate using anti-KIF2C monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with KIF2C rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged KIF2C is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC014924   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q99661 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a member of kinesin-like protein family. Proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. (provided by RefSeq) |
| Immunogen: | KIF2C (AAH14924, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAMDSSLQARLFPGLAIKIQRSNGL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |