Monoclonal Anti-CPSF6 antibody produced in mouse
SIGMA/WH0011052M10 - clone 3F11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CFIM; Anti-CFIM68; Anti-HPBRII4; Anti-HPBRII7; Anti-cleavage and polyadenylation specific factor 6, 68kDa
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to CPSF6 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting CPSF6 monoclonal antibody, clone 3F11. Western Blot analysis of CPSF6 expression in HL-60.
Western Blotting CPSF6 monoclonal antibody, clone 3F11. Western Blot analysis of CPSF6 expression in Raw 264.7.
Western Blotting Western Blot analysis of CPSF6 expression in transfected 293T cell line by CPSF6 monoclonal antibody, clone 3F11. Lanes Lane 1: CPSF6 transfected lysate (59.21 kDa). Lane 2: Non-transfected lysate.
Western Blotting CPSF6 monoclonal antibody, clone 3F11 Western Blot analysis of CPSF6 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged CPSF6 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_007007   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q6NVF9 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is one subunit of a cleavage factor required for 3′ RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3′ end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins. (provided by RefSeq) |
| Immunogen: | CPSF6 (NP_008938, 37 a.a. ~ 136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence ISPSANNGDAPEDRDYMDTLPPTVG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |