Monoclonal Anti-MAP4K5 antibody produced in mouse
SIGMA/WH0011183M1 - clone 3H3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-GCKR; Anti-KHS; Anti-KHS1; Anti-MAPKKKK5; Anti-
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of MAP4K5 over-expressed 293 cell line, cotransfected with MAP4K5 Validated Chimera RNAi. Blot probed with MAP4K5 monoclonal antibody (M01) clone 3H3. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of MAP4K5 expression in transfected 293T cell line by MAP4K5 monoclonal antibody, clone 3H3. Lanes Lane 1: MAP4K5 transfected lysate (95.04 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).
ELISA Detection limit for recombinant GST tagged MAP4K5 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3H3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC036013   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9Y4K4 |
| General description: | This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. (provided by RefSeq) |
| Immunogen: | MAP4K5 (AAH36013, 361 a.a. ~ 470 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SSDPNFMLQWNPFVDGANTGKSTSK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |