Monoclonal Anti-CCT5 antibody produced in mouse
SIGMA/WH0022948M1 - clone 4E5-4B1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CCTE; Anti-CCTepsilon; Anti-KIAA0098; Anti-TCP1epsilon; Anti-chaperonin containing TCP1, subunit 5 (epsilon)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CCT5 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 1 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to CCT5 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting CCT5 monoclonal antibody, clone 4E5-4B1 Western Blot analysis of CCT5 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (85.25 kDa).
ELISA Detection limit for recombinant GST tagged CCT5 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4E5-4B1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC006543   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P48643 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. (provided by RefSeq) |
| Immunogen: | CCT5 (AAH06543, 1 a.a. ~ 541 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MASMGTLAFDEYGRPFLIIKDQDRK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |