Monoclonal Anti-CBX5 antibody produced in mouse
SIGMA/WH0023468M1 - clone 1E11-3A10, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HP1; Anti-HP1ALPHA; Anti-HP1Hsalpha; Anti-chromobox homolog 5 (HP1 alpha homolog, Drosophila)
MDL Number: MFCD08705463
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to CBX5 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting CBX5 monoclonal antibody, clone 1E11-3A10 Western Blot analysis of CBX5 expression in HeLa S3 NE.
Western Blotting Western Blot analysis of CBX5 expression in transfected 293T cell line by CBX5 monoclonal antibody, clone 1E11-3A10. Lanes Lane 1: CBX5 transfected lysate (22.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (46.75 kDa).
ELISA Detection limit for recombinant GST tagged CBX5 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1E11-3A10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC006821   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P45973 |
| General description: | This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. (provided by RefSeq) |
| Immunogen: | CBX5 (AAH06821, 1 a.a. ~ 191 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGKKTKRTADSSSSEDEEEYVVEKV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |