Monoclonal Anti-NIFUN antibody produced in mouse
SIGMA/WH0023479M1 - clone 3B8-1C4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-2310020H20Rik; Anti-ISCU; Anti-ISU2; Anti-MGC74517; Anti-NIFU; Anti-NifU-like N-terminal domain containing
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to NIFUN on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting NIFUN monoclonal antibody, clone 3B8-1C4 Western Blot analysis of NIFUN expression in HL-60.
Western Blotting QC Western Blot detection against Immunogen (41.36 kDa).
ELISA Detection limit for recombinant GST tagged NIFUN is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3B8-1C4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC011906   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9H1K1 |
| General description: | Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria. (provided by RefSeq) |
| Immunogen: | NIFUN (AAH11906, 26 a.a. ~ 167 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence RELSAPARLYHKKVVDHYENPRNVG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |