Monoclonal Anti-NUP62 antibody produced in mouse
SIGMA/WH0023636M2 - clone 2D3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DKFZp547L134; Anti-FLJ20822; Anti-MGC841; Anti-nucleoporin 62kDa; Anti-p62
MDL Number: MFCD01634158
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to NUP62 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting NUP62 monoclonal antibody, clone 2D3 Western Blot analysis of NUP62 expression in HeLa S3 NE.
Western Blotting NUP62 monoclonal antibody, clone 2D3. Western Blot analysis of NUP62 expression in NIH/3T3.
Western Blotting NUP62 monoclonal antibody, clone 2D3. Western Blot analysis of NUP62 expression in PC-12.
Western Blotting Western Blot analysis of NUP62 expression in transfected 293T cell line by NUP62 monoclonal antibody, clone 2D3. Lanes Lane 1: NUP62 transfected lysate (Predicted MW: 53.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged NUP62 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2D3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_153719   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P37198 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. (provided by RefSeq) |
| Immunogen: | NUP62 (NP_714941, 423 a.a. ~ 522 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LQHADEEREKTYKLAENIDAQLKRM |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |