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Monoclonal Anti-SAMM50 antibody produced in mouse

SIGMA/WH0025813M4 - clone 2A9, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-CGI51; Anti-OMP85; Anti-SAM50; Anti-TOB55; Anti-TRG3; Anti-YNL026W; Anti-sorting and assembly machinery component 50 homolog (S. cerevisiae)

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-WH0025813M4-100UG 100 µg
$524.00
1/EA
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Western Blotting SAMM50 monoclonal antibody, clone 2A9 Western Blot analysis of SAMM50 expression in A-431.
Western Blotting QC Western Blot detection against Immunogen (77.22 kDa).

 

antibody form purified immunoglobulin
antibody product type primary antibodies
biological source mouse
clone 2A9, monoclonal
conjugate unconjugated
form buffered aqueous solution
GenBank® accession no. BC007830 
isotype IgG2bκ
Quality Level 100 
shipped in dry ice
species reactivity mouse
storage temp. −20°C
technique(s) indirect ELISA: suitable
  western blot: 1-5 μg/mL
UniProt accession no. Q8BGH2 
Biochem/physiol Actions: Sorting and assembly machinery (SAM) 50 helps in aggregation of β-barrel proteins into the outer mitochondrial membrane (OMM). SAM 50, along with mitofilin and coiled-coil-helix-coiled-coil-helix domain containing 3 (CHCHD3), plays a vital role in maintaining mitochondrial shape, the morphology of cristae, and the assembly of respiratory complexes. Elevated expression of SAMM50 leads to mitochondrial fragmentation in HeLa cells under the influence of dynamin-related protein (Drp) 1. Depleted expression of SAM 50 affects assembly and steady-state level of voltage-dependent anion-selective channel (VDAC), which is essential for transport of metabolite across OMM.
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description: SAMM50 is a component of the sorting and assembly machinery (SAM) complex of the outer mitochondrial membrane. The SAM complex has a role in integrating beta-barrel proteins into the outer mitochondrial membrane (Humphries et al., 2005 [PubMed 15644312]).[supplied by OMIM
Immunogen: SAMM50 (AAH07830, 1 a.a. ~ 468 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
MGTVHARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLIDTCQGDDALPNGLDVTFEVTELRRLTGSYNTMVGNNEGSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPRPGNFERNFSVNLYKVTGQFPWSSLRETDRGMSAEYSFPIWKTSHTVKWEGVWRELGCLSRTASFAVRKESGHSLKSSLSHAMVIDSRNSSILPRRGALLKVNQELAGYTGGDVSFIKEDFELQLNKQLIFDSVFSASFWGGMLVPIGDKPSSIADRFYLGGPTSVRGFSMHSIGPQSEGDYLGGEAYWAGLHLYTPLPFRPGQGGFGELFRTHFFLNAGNLCNLNYGEGPKAHIRKLAECIRWSYGAGIVLRLGNIARLELNYCVPMGVQTGDRICDGVQFGAGIRFL
Legal Information: GenBank is a registered trademark of United States Department of Health and Human Services
Physical form: Solution in phosphate buffered saline, pH 7.4
RIDADR NONH for all modes of transport
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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