Monoclonal Anti-LDLRAP1 antibody produced in mouse
SIGMA/WH0026119M1 - clone 4G4-D5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ARH; Anti-ARH2; Anti-DKFZp586D0624; Anti-FHCB1; Anti-FHCB2; Anti-MGC34705; Anti-low density lipoprotein receptor adaptor protein 1
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to LDLRAP1 on formalin-fixed paraffin-embedded human maligant fibrous histiocytoma tissue. [antibody concentration 2 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to LDLRAP1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting LDLRAP1 monoclonal antibody, clone 4G4-D5 Western Blot analysis of LDLRAP1 expression in HeLa S3 NE.
Western Blotting Western Blot analysis of LDLRAP1 expression in transfected 293T cell line by LDLRAP1 monoclonal antibody, clone 4G4-D5. Lanes Lane 1: LDLRAP1 transfected lysate (29 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (54.67 kDa).
ELISA Detection limit for recombinant GST tagged LDLRAP1 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4G4-D5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC029770   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q5SW96 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia. (provided by RefSeq) |
| Immunogen: | LDLRAP1 (AAH29770, 1 a.a. ~ 263 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MLFSLKYLGMTLVEQPKGEELSAAA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |