Monoclonal Anti-PTPN22 antibody produced in mouse
SIGMA/WH0026191M1 - clone 4F6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-LYP; Anti-Lyp1; Anti-Lyp2; Anti-PEP; Anti-PTPN8; Anti-protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
MDL Number: MFCD00151733
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of PTPN22 over-expressed 293 cell line, cotransfected with PTPN22 Validated Chimera RNAi. Blot probed with PTPN22 monoclonal antibody, clone 4F6. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of PTPN22 expression in transfected 293T cell line by PTPN22 monoclonal antibody, clone 4F6. Lanes Lane 1: PTPN22 transfected lysate (85.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (45.43 kDa).
ELISA Detection limit for recombinant GST tagged PTPN22 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC017785   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9Y2R2 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves′ disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. (provided by RefSeq) |
| Immunogen: | PTPN22 (AAH17785, 1 a.a. ~ 179 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDQREILQKFLDEAQSKKITKEEFA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |