Monoclonal Anti-PHGDH antibody produced in mouse
SIGMA/WH0026227M1 - clone 4A3-1D6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-3PGDH; Anti-MGC3017; Anti-PDG; Anti-PGAD; Anti-PGD; Anti-PGDH; Anti-SERA; Anti-
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 1 ~ 10 μg/mL]
Immunohistochemistry Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to PHGDH on HeLa cell. [antibody concentration 1 ~ 10 μg/mL]
Western Blotting PHGDH monoclonal antibody, clone 4A3-1D6. Western Blot analysis of PHGDH expression in human liver.
Western Blotting PHGDH monoclonal antibody, clone 4A3-1D6 Western Blot analysis of PHGDH expression in Jurkat.
Western Blotting Western Blot analysis of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody, clone 4A3-1D6. Lanes Lane 1: PHGDH transfected lysate (56.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting PHGDH monoclonal antibody, clone 4A3-1D6. Western Blot analysis of PHGDH expression in A-431.
Western Blotting QC Western Blot detection against Immunogen (84.37 kDa).
Immunoprecipitation Immunoprecipitation of PHGDH transfected lysate using anti-PHGDH monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PHGDH rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4A3-1D6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC011262   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O43175 |
| Application: | Monoclonal Anti-PHGDH antibody produced in mouse has been used in Western Blotting. |
| Biochem/physiol Actions: | The gene PHGDH (D-3-phosphoglycerate dehydrogenase) is mapped to human chromosome 1p. The enzyme is mainly responsible for catalyzing initial step of the serine synthesis pathway, converting 3-phosphoglycerate into 3-phosphohydroxypyruvate. It is up-regulated in estrogen receptor-negative breast cancers, melanoma and cervical cancers. Mutations in the gene encoding PHGDH are associated with Neu-Laxova syndrome. Down-regulation in PHGDH levels results in congenital microcephaly, psychomotor retardation and seizures. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | PHGDH (AAH11262.1, 1 a.a. ~ 533 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAFANLRKVLISDSLDPCCRKILQD |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |