Monoclonal Anti-STEAP1 antibody produced in mouse
SIGMA/WH0026872M1 - clone 4F6-1F3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MGC19484; Anti-PRSS24; Anti-STEAP; Anti-six transmembrane epithelial antigen of the prostate 1
MDL Number: MFCD03097291
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to STEAP1 on A-431 cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of STEAP1 expression in transfected 293T cell line by STEAP1 monoclonal antibody, clone 4F6-1F3. Lanes Lane 1: STEAP1 transfected lysate (Predicted MW: 39.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting STEAP1 monoclonal antibody, clone 4F6-1F3 Western Blot analysis of STEAP1 expression in A-431.
Western Blotting QC Western Blot detection against Immunogen (63.03 kDa).
ELISA Detection limit for recombinant GST tagged STEAP1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F6-1F3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC011802   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9UHE8 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions. (provided by RefSeq) |
| Immunogen: | STEAP1 (AAH11802, 1 a.a. ~ 339 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MESRKDITNQEELWKMKPRRNLEED |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |