Monoclonal Anti-CACYBP antibody produced in mouse
SIGMA/WH0027101M1 - clone 2E3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-GIG5; Anti-MGC87971; Anti-PNAS107; Anti-RP1102G20.6; Anti-S100A6BP; Anti-SIP; Anti-calcyclin binding protein
MDL Number: MFCD04040491
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between SKP1 and CACYBP. HeLa cells were stained with anti-SKP1 rabbit purified polyclonal 1:1200 and anti-CACYBP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting Western Blot analysis of CACYBP expression in transfected 293T cell line by CACYBP monoclonal antibody, clone 2E3. Lanes Lane 1: CACYBP transfected lysate (26.61 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (50.82 kDa).
ELISA Detection limit for recombinant GST tagged CACYBP is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC005975   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9HB71 |
| Biochem/physiol Actions: | CACYBP (calcyclin binding protein) is a part of the ubiquitination machinery and is involved in the degradation of β-catenin. It can interact with S100, SIAH1 (seven in absentia homolog 1) and SKP1 (S-phase kinase-associated protein 1). CACYBP is involved in proliferation, differentiation, multidrug resistance and tumorigenicity. It can work as an oncogene and as a tumor suppressor. In glioma cells, CACYBP enhances the cellular proliferation. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | CACYBP (AAH05975, 1 a.a. ~ 228 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MASEELQKDLEEVKVLLEKATRKRV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |