Monoclonal Anti-GOLPH4 antibody produced in mouse
SIGMA/WH0027333M1 - clone 5E11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-GIMPC; Anti-GPP130; Anti-P138; Anti-golgi phosphoprotein 4
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to GOLPH4 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting GOLPH4 monoclonal antibody, clone 5E11 Western Blot analysis of GOLPH4 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (36.3 kDa).
ELISA Detection limit for recombinant GST tagged GOLPH4 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5E11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_014498   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O00461 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi-resident protein. It may process proteins synthesized in the rough endoplasmic reticulum and assist in the transport of protein cargo through the Golgi apparatus. (provided by RefSeq) |
| Immunogen: | GOLPH4 (NP_055311.1, 473 a.a. ~ 568 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence HQEQLRQQAHYDAMDNDIVQGAEDQ |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |