Monoclonal Anti-NXT1 antibody produced in mouse
SIGMA/WH0029107M8 - clone 4F11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MTR2; Anti-NTF2-like export factor 1; Anti-P15
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to NXT1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of NXT1 expression in transfected 293T cell line by NXT1 monoclonal antibody, clone 4F11. Lanes Lane 1: NXT1 transfected lysate (15.51 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of NXT1 over-expressed 293 cell line, cotransfected with NXT1 Validated Chimera RNAi. Blot probed with NXT1 monoclonal antibody, clone 4F11. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (41.14 kDa).
ELISA Detection limit for recombinant GST tagged NXT1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC000759   |
| isotype | IgG1λ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9UKK6 |
| General description: | The protein encoded by this gene is located in the nuclear envelope. It has protein similarity to nuclear transport factor 2. This protein functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. The encoded protein heterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export. The use of alternate polyadenylation sites has been found for this gene. (provided by RefSeq) |
| Immunogen: | NXT1 (AAH00759, 1 a.a. ~ 140 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MASVDFKTYVDQACRAAEEFVNVYY |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |