Monoclonal Anti-EHD2 antibody produced in mouse
SIGMA/WH0030846M1 - clone 2D8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-EH-domain containing 2; Anti-PAST2
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to EHD2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of EHD2 expression in transfected 293T cell line by EHD2 monoclonal antibody, clone 2D8. Lanes Lane 1: EHD2 transfected lysate (61.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting EHD2 monoclonal antibody, clone 2D8 Western Blot analysis of EHD2 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (32.67 kDa).
ELISA Detection limit for recombinant GST tagged EHD2 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2D8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_014601   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9NZN4 |
| General description: | This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. (provided by RefSeq) |
| Immunogen: | EHD2 (NP_055416, 365 a.a. ~ 427 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence AHDFTKFHSLKPKLLEALDEMLTHD |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |