Monoclonal Anti-IL20 antibody produced in mouse
SIGMA/WH0050604M1 - clone 2H8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-IL10D; Anti-IL20; Anti-MGC96907; Anti-ZCYTO10; Anti-interleukin 20
MDL Number: MFCD05665228
Product Type: Chemical
Western Blotting Western Blot analysis of IL20 expression in transfected 293T cell line by IL20 monoclonal antibody, clone 2H8. Lanes Lane 1: IL20 transfected lysate (20.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting IL20 monoclonal antibody, clone 2H8. Western Blot analysis of IL20 expression in A-431.
Western Blotting IL20 monoclonal antibody, clone 2H8 Western Blot analysis of IL20 expression in K-562.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
Immunoprecipitation Immunoprecipitation of IL20 transfected lysate using anti-IL20 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with IL20 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged IL20 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2H8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_018724   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9NYY1 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. (provided by RefSeq) |
| Immunogen: | IL20 (NP_061194, 67 a.a. ~ 176 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence RTESLQDTKPANRCCLLRHLLRLYL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |