Monoclonal Anti-F11R antibody produced in mouse
SIGMA/WH0050848M1 - clone 2E3-1C8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-F11 receptor; Anti-JAM; Anti-JAM1; Anti-JAMA; Anti-JCAM; Anti-KAT; Anti-PAM1
MDL Number: MFCD04118557
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to F11R on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 1 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between PRKCZ and F11R. HeLa cells were stained with anti-PRKCZ rabbit purified polyclonal 1:1200 and anti-F11R mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting Western Blot analysis of F11R expression in transfected 293T cell line by F11R monoclonal antibody, clone 2E3-1C8. Lanes Lane 1: F11R transfected lysate (Predicted MW: 32.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting F11R monoclonal antibody, clone 2E3-1C8 Western Blot analysis of F11R expression in HepG2.
Western Blotting QC Western Blot detection against Immunogen (58.63 kDa).
ELISA Detection limit for recombinant GST tagged F11R is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E3-1C8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC001533   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9Y624 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5′ alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. (provided by RefSeq) |
| Immunogen: | F11R (AAH01533, 1 a.a. ~ 299 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGTKAQVERKLLCLFILAILLCSLA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |