Monoclonal Anti-RNF141 antibody produced in mouse
SIGMA/WH0050862M1 - clone 6D9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MGC8715; Anti-ZFP26; Anti-ZNF230; Anti-ring finger protein 141
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to RNF141 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.3 μg/mL]
Western Blotting RNF141 monoclonal antibody, clone 6D9. Western Blot analysis of RNF141 expression in PC-12.
Enhanced Validation-RNAi Western blot analysis of RNF141 over-expressed 293 cell line, cotransfected with RNF141 Validated Chimera RNAi. Blot probed with RNF141 monoclonal antibody, clone 6D9. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting RNF141 monoclonal antibody, clone 6D9 Western Blot analysis of RNF141 expression in A-431.
Western Blotting RNF141 monoclonal antibody, clone 6D9. Western Blot analysis of RNF141 expression in Raw 264.7.
Western Blotting Western Blot analysis of RNF141 expression in transfected 293T cell line by RNF141 monoclonal antibody, clone 6D9. Lanes Lane 1: RNF141 transfected lysate (25.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (35.53 kDa).
ELISA Detection limit for recombinant GST tagged RNF141 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6D9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_016422   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q8WVD5 |
| General description: | The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-DNA and protein-protein interactions. Abundant expression of this gene was found in the testicular tissue of fertile men, but was not detected in azoospermic patients. Studies of the mouse counterpart suggest that this gene may function as a testis specific transcription factor during spermatogenesis. (provided by RefSeq) |
| Immunogen: | RNF141 (NP_001008563, 141 a.a. ~ 229 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LWMGRVKQLTDEEECCICMDGRADL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |