Monoclonal Anti-ANAPC11 antibody produced in mouse
SIGMA/WH0051529M1 - clone 1B4-1A4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-APC11; Anti-APC11 anaphase promoting complex subunit 11 homolog (yeast); Anti-Apc11p; Anti-HSPC214; Anti-MGC882
MDL Number: MFCD03454736
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between CDC20 and ANAPC11. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-ANAPC11 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting QC Western Blot detection against Immunogen (31.68 kDa).
ELISA Detection limit for recombinant GST tagged ANAPC11 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B4-1A4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC000607   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9NYG5 |
| Biochem/physiol Actions: | ANAPC11 (Anaphase promoting complex subunit 11) plays a key role in cell division by accelerating ubiquitination of cell-cycle regulators such as cyclin B and securin. It acts as the catalytic subunit of anaphase-promoting complex (APC) and helps to translocate ubiquitin from a ubiquitin-conjugating enzyme (E2) to the substrate. It controls the cell cycle progression through G1 phase of the cell cycle during protein modification. |
| General description: | ANAPC11 (Anaphase promoting complex subunit 11) is a E3 ubiquitin ligase consisting of a RING-H2-finger domain with one histidine and seven cysteine residues that coordinate two Zn(2+) ions. It is mapped on the human chromosome 17q25 and is expressed in the cytoplasm and nucleus with discrete accumulation in granular structures in all the cell lines (AML 12, HepG2, and C2C12) transfected. |
| Immunogen: | ANAPC11 (AAH00607, 1 a.a. ~ 54 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGPGPVGKVPGDDCPLVWGQCSHCF |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |