Monoclonal Anti-KRT20 antibody produced in mouse
SIGMA/WH0054474M1 - clone 2G3-1C8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CK20; Anti-K20; Anti-KRT21; Anti-MGC35423; Anti-keratin 20
MDL Number: MFCD02263408
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to KRT20 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to KRT20 on HeLa cell. [antibody concentration 10 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to KRT20 on U-2 OS cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of KRT20 expression in transfected 293T cell line by KRT20 monoclonal antibody, clone 2G3-1C8. Lanes Lane 1: KRT20 transfected lysate (48.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting KRT20 monoclonal antibody, clone 2G3-1C8 Western Blot analysis of KRT20 expression in A-431.
Western Blotting QC Western Blot detection against Immunogen (72.38 kDa).
ELISA Detection limit for recombinant GST tagged KRT20 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2G3-1C8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC031559   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P35900 |
| Biochem/physiol Actions: | KRT20 (keratin 20) helps in intermediate filament organization. In metastatic colorectal cancer, it can be used as a biomarker for checking circulating tumor cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. (provided by RefSeq) |
| Immunogen: | KRT20 (AAH31559, 1 a.a. ~ 424 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDFSRRSFHRSLSSSLQAPVVSTVG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |