Monoclonal Anti-UBE2R2 antibody produced in mouse
SIGMA/WH0054926M1 - clone 5E7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CDC34B; Anti-FLJ20419; Anti-MGC10481; Anti-UBC3B; Anti-
Product Type: Chemical
Western Blotting Western Blot analysis of UBE2R2 expression in transfected 293T cell line by UBE2R2 monoclonal antibody, clone 5E7. Lanes Lane 1: UBE2R2 transfected lysate (Predicted MW: 27.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting UBE2R2 monoclonal antibody, clone 5E7 Western Blot analysis of UBE2R2 expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (51.92 kDa).
Immunoprecipitation Immunoprecipitation of UBE2R2 transfected lysate using anti-UBE2R2 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with UBE2R2 monoclonal antibody.
ELISA Detection limit for recombinant GST tagged UBE2R2 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5E7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC047584   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q712K3 |
| General description: | Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. (provided by RefSeq) |
| Immunogen: | UBE2R2 (AAH47584, 1 a.a. ~ 238 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAQQQMTSSQKALMLELKSLQEEPV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |