Monoclonal Anti-SSH3 antibody produced in mouse
SIGMA/WH0054961M1 - clone 6F9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FLJ10928; Anti-FLJ20515; Anti-SSH3; Anti-slingshot homolog 3 (Drosophila)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SSH3 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 1 ~ 10 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to SSH3 on A-431 cell. [antibody concentration 10 μg/mL]
Western Blotting SSH3 monoclonal antibody, clone 6F9 Western Blot analysis of SSH3 expression in A-431.
Western Blotting Western Blot analysis of SSH3 expression in transfected 293T cell line by SSH3 monoclonal antibody, clone 6F9. Lanes Lane 1: SSH3 transfected lysate (73 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
Immunoprecipitation Immunoprecipitation of SSH3 transfected lysate using anti-SSH3 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with SSH3 monoclonal antibody.
ELISA Detection limit for recombinant GST tagged SSH3 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6F9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_017857   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q8TE77 |
| Biochem/physiol Actions: | SSH3 (Slingshot protein phosphatase 3) is involved in the actin filament assembly. During actin reorganization, LIM-kinase 1 (LIMK1) restricts the activity of ADF (actin-depolymerizing factor)/cofilin, which is a stimulus-responsive mediator of actin dynamics. SSH3 blocks the LIMK1 functions and reactivates ADF/cofilin in vivo by the dephosphorylating it. In addition, it is also involved in several cellular and developmental activities. |
| General description: | The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM |
| Immunogen: | SSH3 (NP_060327, 293 a.a. ~ 391 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SKEIRQALELRLGLPLQQYRDFIDN |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |