Monoclonal Anti-LRRC8D antibody produced in mouse
SIGMA/WH0055144M1 - clone 3H1-1C2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FLJ10470; Anti-FLJ20403; Anti-leucine rich repeat containing 5
Product Type: Chemical
Western Blotting Western Blot analysis of LRRC8D expression in transfected 293T cell line by LRRC5 monoclonal antibody, clone 3H1-1C2. Lanes Lane 1: LRRC8D transfected lysate (16 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (41.47 kDa).
ELISA Detection limit for recombinant GST tagged LRRC8D is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3H1-1C2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC009486   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q7L1W4 |
| Biochem/physiol Actions: | Leucine rich repeat containing 8 VRAC subunit D (LRRC8D) is important for the translocation of neutral compounds like myo-inositol, taurine, γ-aminobutyric acid (GABA) and prolonged transport of positively charged lysine. LRRC8D deficiency is associated with reduced cellular platinum level. The protein acts as a transporter of small molecules. It is essential for the import of the antibiotic blasticidin S. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Leucine rich repeat containing 8 VRAC subunit D (LRRC8D) is a mammalian protein, which belongs to the family of leucine-rich repeat-containing 8 (LRRC8). It consists of four transmembrane domains at the N terminal and 17 leucine-rich repeats (LRR) at the C terminal. The gene is located on human chromosome 1p22.2. |
| Immunogen: | LRRC5 (AAH09486, 1 a.a. ~ 143 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MVSSNFWFKYPKTCSKVEHFVSILG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |