Monoclonal Anti-THAP1 antibody produced in mouse
SIGMA/WH0055145M1 - clone 2C1-2F2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FLJ10477; Anti-MGC33014; Anti-THAP domain containing, apoptosis associated protein 1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to THAP1 on HeLa cell. [antibody concentration 10 μg/mL]
Enhanced Validation-RNAi Western blot analysis of THAP1 over-expressed 293 cell line, cotransfected with THAP1 Validated Chimera RNAi. Blot probed with THAP1 monoclonal antibody, clone 2C1-2F2. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of THAP1 expression in transfected 293T cell line by THAP1 monoclonal antibody, clone 2C1-2F2. Lanes Lane 1: THAP1 transfected lysate (24.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (49.17 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2C1-2F2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC021721   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9NVV9 |
| Biochem/physiol Actions: | THAP domain containing 1 (THAP1) plays a key role in endothelial cell proliferation and pro-apoptotic processes. Mutation in the gene is associated with primary dystonia type 6 (DYT6). The encoded protein links prostate-apoptosis-respon |
| General description: | THAP domain containing 1 (THAP1) is a transcription factor encoded by the gene mapped to human chromosome 18p11.21. The encoded protein contains an N-terminal conserved DNA-binding domain, designated THAP domain with a zinc-finger structure. |
| Immunogen: | THAP1 (AAH21721, 1 a.a. ~ 213 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MVQSCSAYGCKNRYDKDKPVSFHKF |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |