Monoclonal Anti-CHFR antibody produced in mouse
SIGMA/WH0055743M1 - clone 1H3-A12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FLJ10796; Anti-RNF116; Anti-RNF196; Anti-checkpoint with forkhead and ring finger domains
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 μg/mL]
Enhanced Validation-RNAi Western blot analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi. Blot probed with CHFR monoclonal antibody, clone 1H3-A12. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody, clone 1H3-A12. Lanes Lane 1: CHFR transfected lysate (Predicted MW: 72.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting CHFR monoclonal antibody, clone 1H3-A12. Western Blot analysis of CHFR expression in 293.
Western Blotting QC Western Blot detection against Immunogen (97.46 kDa).
ELISA Detection limit for recombinant GST tagged CHFR is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1H3-A12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC012072   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL using HeLa cell lysate | |
| UniProt accession no. | Q96EP1 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | CHFR (AAH12072.1, 1 a.a. ~ 652 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MERPEEGKQSPPPQPWGRLLRLGAE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |