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Monoclonal Anti-DIABLO antibody produced in mouse

SIGMA/WH0056616M2 - clone 4F9, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-DIABLOS; Anti-FLJ10537; Anti-FLJ25049; Anti-SMAC; Anti-SMAC3; Anti-diablo homolog (Drosophila)

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-WH0056616M2-50UG 50 µg
$524.00
1/EA
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Western Blotting Western Blot analysis of DIABLO expression in transfected 293T cell line by DIABLO monoclonal antibody, clone 4F9. Lanes Lane 1: DIABLO transfected lysate (27.131 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged DIABLO is approximately 0.3 ng/mL as a capture antibody.

 

antibody form purified immunoglobulin
antibody product type primary antibodies
biological source mouse
clone 4F9, monoclonal
conjugate unconjugated
form buffered aqueous solution
GenBank® accession no. BC011909 
isotype IgG2aκ
Quality Level 100 
shipped in dry ice
species reactivity human
storage temp. −20°C
technique(s) indirect ELISA: suitable
  western blot: 1-5 μg/mL
UniProt accession no. Q9NR28 
General description: This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. (provided by RefSeq)
Immunogen: DIABLO (AAH11909, 119 a.a. ~ 218 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
SLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQ
Legal Information: GenBank is a registered trademark of United States Department of Health and Human Services
Physical form: Solution in phosphate buffered saline, pH 7.4
RIDADR NONH for all modes of transport
WGK Germany nwg
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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